Select™ Series UV Crosslinkers

High Quality at An Affordable Price!

• Covalently Binds Nucleic-Acid Samples to Membranes in Under 30 Seconds – 240 times faster than vacuum-oven baking!
  
• Safeguards Your Valuable Test Results from Washouts – unique true-UV-monitoring circuitry assures that the set dosage is always delivered
  
• Ensures Optimum Membrane Binding – even as tubes age!
  
• “Smart” Microprocessor Controller Provides Unequalled Ease of Use – auto repeat, “help” prompts and four operation modes (including automatic optimal crosslink) make error-free results routine
  
• Typical Intensity of XLE-1000 is 3,500-4,500 µW/cm²

Versatile – Numerous Applications include:

• Elimination of PCR contamination^8,9,10
• Nicking ethidium-bromide-stained DNA in agarose gels¹¹
• Gene mapping for creating cleavage-inhibiting thymine dimers¹²
• RecA mutation screening in E. coli³
• UV irradiation^13,14,15
• UV-intensity monitoring
• Miscellaneous UV-dosage applications including UV-induced polymerization or drying

Programmable, User-Friendly, “Smart” Microprocessor Controller Makes the Select™ Series UV Crosslinkers Simple and Foolproof!

Built-In “Help” Messages and LED-Function Indicators Guide You Every Step of the Way, Reminding You of:

• Selected Settings
• Door Open
• Remaining Energy
• End of Cycle
• When Tubes Need Replacement

Auto Repeat “Remembers” Previous Settings for Quick and Simple, One-Button Operation. You never have to enter the same numbers over and over. Just press “Start” to automatically repeat or to resume a user-interrupted operation. Should the door be opened during operation, the XLE-1000 crosslinker alternately flashes “door” and the remaining time or energy. The “Reset” button cancels the latest settings.

Unparalleled Accuracy – Our state-of-the-art photo
sensor provides true UV monitoring. Competitors’ units
employ inferior, bare photodiodes, which render them inaccurate
by reading unwanted visible and infrared radiations.
The XLE-1000 crosslinker’s exclusive, wavelength-specific,
multi-stack UV photo sensor, which is factory-calibrated to
NIST (NBS) specifications, provides the most precise UV-only
dosage measurements. 254nm tubes are standard,
with 312nm or 365nm tubes optional. Full-range-display
resolution is accurate to ±5µW/cm2. EMI/RFI protection.

Super-Fast Crosslinking – Covalently Binds Samples to Membranes in Under 30 Seconds. Vacuum-oven baking would take over two hours at 80°C. The crosslinker is over 240 times faster!

Safeguards Your Valuable Test Results and Optimizes Binding. After investing your valuable time and effort, the last thing you want is to have your improperly bound sample disappear during the wash cycle. No need to worry about that with the XLE-1000 crosslinker! Just select the desired energy level and its unique true-UV-monitoring circuitry assures that the dosage you set is always precisely delivered. Even when the tubes age or if the AC-line power fluctuates, the crosslinker’s automatic photoelectric
feedback circuitry assures that your samples will be properly irradiated. And its “smart” microprocessor controller, large LED display and color-coded key pad reduce human error. An anodized-aluminum chamber prevents sample contamination due to rust.

References

1. Manufactured by and Trademark of DuPont New England Nuclear, Boston, MA
2. Manufcatured by Schleicher & Schuell, Keene, NH
3. J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, 2nd Edition, 1989.
4. Manufactured by Ambis Systems, San Diego, CA
5. G.M. Church, W. Gilbert, Proceedings of National Academy of Sciences/USA, 81: 1991-1995, 1984.
6. E.W. Khandjian, Biotechnology, 5: 165-167, 1987.
7. E.W. Khandjian, Molecular Biology Reports, 11: 107-115, 1986.
8. G. Sarkar, S.S. Somer, Nature, 343: 27, 1990.
9. G.D. Cimino, K. Metchette, S.T. Isaacs, U.S. Zhu, Nature, 345: 773-774, 1990.
10. C. Ou, J.L. Moore, G. Schochetman, Biotechniques, 10: 442-445, 1991.
11. D. Vollrath, R.W. Davis, Nucleic Acid Research, 15: 7865-7876, 1987.
12. P.A. Whittaker, E.M. Southern, Gene, 41: 129-134, 1986.
13. R.R. Reich, H.D. Anderson, "Sterilization of Membrane Filters with Ultraviolet Irradiation," Pharmaceutical Manufacturing, 12-15, 1986.
14. R.R. Reich, H.D. Anderson, "Laboratory Applications of Ultraviolet Irradiation," Medical Device & Diagnostic Industry, 52-58, 1985.
15. L.R. Koller, Ultraviolet Radiation, 236-245, John Wiley & Sons, 1965.