The Ideal Cassettes and Screens for Autoradiography

In autoradiography, a radioactively labeled probe is hybridized with a specimen material which is contained in a gel (polyacrylamide or agarose) or on a membrane (nylon or nitrocellulose). The hybridized specimen is then placed inside a cassette where it is in contact with a high-speed X-ray film. After the cassette is stored at a temperature of -70°C for a few days, the radioactivity forms an image representing the spatial distribution of the sample under investigation.

 

Rugged Spectroline FOUR SQUARE^® cassettes are the undisputed choice for maximum strength.

These cassettes are built with stainless-steel, closed-channel structure girders, spot-welded interior flanges and butt-welded exterior corners. In addition to being extremely sturdy with a .005" thick, lead-lined door, these cassettes have a phenolic panel laminated to an aluminum alloy plate so they will not warp or corrode. Their hinges are tough enough to endure constant, everyday use and a freezer temperature of -70°C.

The FOUR SQUARE models are designed with stainless-steel locking bars for excellent closure and protection from accidental opening. For convenient darkroom handling, a corrosion-resistant ‘‘D’’ ring is provided for easy opening of the cassette door.

The hinges, locking bars and front panel of the FOUR SQUARE cassettes work in combination to provide the necessary pressure at all points for perfect film/screen contact. The hinges’ ‘‘floating action’’ design allows the use of any type or thickness of intensifying screen. Unmatched film/screen contact provides quicker, more distinct autoradiographic images.

 

Economical VALUE cassettes have features comparable to higher-priced models.

The VALUE cassettes have a lightweight, aluminum construction. They feature durable push-button latches and a spring-loaded door for easy opening. Resilient polymer corners are designed to protect against dam- age when dropped.

 

 

Spectroline L-PLUS^® intensifying screens increase productivity by providing faster, sharper autoradiographic images.

To reduce exposure time, use one or two L-PLUS screens. Their high absorption and low noise also sharpen image contrast.